DSpace

Future University Hakodate Academic Archive >
Faculty and Students >
Dept. of Complex and Intelligent Systems >
Nakagaki Toshiyuki >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10445/4392

Title: Kinetic study of anti-viral ribavirin uptake mediated by hCNT3 and hENT1 in Xenopus laevis oocytes
Authors: Yamamoto, T.
Mitsuru, S.
Takashi, K.
Seiji, M.
Yamaguchi, M.
Tero, A.
Takagi, S.
Nakagaki, Toshiyuki
Abstract: Transport across the cell membrane is crucial in drug delivery. However, the process is complicated because nucleoside derivatives that are commonly used as anti-viral drugs are transported through two different types of specific transporters: concentrative transporters and equilibrative transporters. Cross-disciplinary approaches involving both biological experiments and theoretical considerations are therefore necessary to study the transport of nucleoside analogues such as ribavirin. Here we constructed an experimental model system using the Xenopus laevis oocyte that expressed examples of both types of transporters: human concentrative nucleoside transporter 3 and human equilibrative transporter 1. We also performed a kinetic study. Experimental results showed that the transport of ribavirin could be reduced by inhibiting one of the two types of transporters, which seems to be counterintuitive. We therefore designed a simple mathematical model of the dynamics of ribavirin uptake and analyzed the model behaviors using a numerical simulation. The theoretical results reproduced the experimentally observed phenomena and suggested a possible mechanism for the process. Based on this mechanism, we predicted some potential methods for the effective uptake of ribavirin from a dynamics point of view.
Research Achievement Classification: 原著論文/Original Paper
Type: Journal Article
Peer Review: あり/yes
Solo/Joint Author(s): 共著/joint
Published journal or presented
academic conference: 
Biophysical Chemistry
Volume: 147
Spage: 59
Epage: 65
Date: 2010
Appears in Collections:Nakagaki Toshiyuki

Files in This Item:

File Description SizeFormat
Rivav20091225BioPhysChemRevised.pdf396.56 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Copyright © 2010-2012 FUTURE UNIVERSITY HAKODATE.
DSpace Software Copyright © 2002-2010  Duraspace